Needle-Free Dermal Delivery of a Diphtheria Toxin CRM197 Mutant on Potassium-Doped Hydroxyapatite Microparticles

25 March 2015

Weissmueller NT, Schiffter HA, Carlisle RC, Rollier CS, Pollard AJ. Needle-free dermal delivery of diphtheria toxin CRM197 mutant on potassium-doped hydroxyapatite microparticles. Clin Vaccine Immunol 2015 Mar 25. pii: CVI.00121-15

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Injections with a hypodermic needle and syringe (HNS) are the current standard of care globally, but the use of needles is not without limitation. While a plethora of needle-free injection devices exist, vaccine reformulation is costly and presents a barrier to their widespread clinical application. To provide a simple, needle-free, and broad-spectrum protein antigen delivery platform, the authors of the study developed novel potassium-doped hydroxyapatite (K-Hap) microparticles with improved protein loading capabilities that can provide sustained local antigen presentation and release. K-Hap showed increased protein adsorption over regular hydroxyapatite (P < 0.001), good structural retention of the model antigen (CRM197) with 1% decrease in -helix content and no change in -sheet content upon adsorption, and sustained release in vitro. Needle-free intradermal powder inoculation with K-Hap– CRM197 induced significantly higher IgG1 geometric mean titers (GMTs) than IgG2a GMTs in a BALB/c mouse model (P < 0.001) and induced IgG titer levels that were not different from the current clinical standard (P > 0.05), namely, alum-adsorbed CRM197 by intramuscular (i.m.) delivery. The presented results suggest that K-Hap microparticles may be used as a novel needlefree delivery vehicle for some protein antigens.